Thrust Area D:  Chemistry and Catalysis

 

Accomplishments  11/1/99-7/31/00

 

Thrust Area D is off to a strong start.  Numerous one-on-one discussions among the various participants are ongoing. Video-teleconferenced group meetings have started to trigger cross-fertilization of ideas among the various researchers, especially among students and post-docs from the various research groups within Thrust Area D, but more interaction with other Thrust Areas is needed.  We have initiated mechanisms to foster this type of inter-Area dialog.

 

Benign Synthesis of Chemicals Using Novel  Heterogeneous Catalysts in SCF CO2 (23)  Adewuyi’s group has completed a literature survey of zeolites for heterogeneous organic catalysis in conventional solvents and supercritical media and has shared this data base within the CERSP.  Work on the design, acquisition and set-up of the experimental systems have been initiated.   In collaboration with DeSimone’s group, equipment have been identified which will be used for preliminary batch studies at UNC–CH.  We are working with Engelhard Corporation for supply of commercial zeolite catalysts and possible supply of special zeolite catalysts.  We are developing collaborative research plans with P. Kropp (UNC-CH).  We have ordered a GC/MS system with accessories to analyze products of reactions and elucidate kinetics and reaction mechanisms.

 

Biocatalytic Processes in CO2 Using Hyperthermophylic Enzymes (24)  Kelly has focused on biocatalysis in CO2 using enzymes from hyperthermophilic and mesophilic sources, focusing on nucleotide/nucleoside chemistry in CO2.  We have catalyzed the regioselective esterification of thymidine to the 3’ or 5’ thymidine acetate using an esterase from the hyperthermophilic archaeon S solfataricus. Other reactions of potential pharmaceutical intermediates are being considered. The effect of co-solvents on esterification reactions in water-organic mixtures were studied to help us formulate co-solvent systems for CO2-based biotransformations; e.g., hyperthermophilic phospholipase may be used in the recovery of Naproxen, from racemic mixtures by selective, hydrolytic modification of one stereoisomer. The gene corresponding to this lipase was identified in the Pyrococcus furiosus genome and is being cloned and expressed in E. coli. Preliminary results look promising. In related work, a mesophilic lipase is optimally active in the near critical regime. The mechanism for this phenomenon is being considered. This result will be used to determine optimal conditions for hyperthermophilic enzymes in CO2.

 

Surface-Mediated Reactivity (25)  Kropp has found that selective oxidation of amines (I or II) to hydroxylamines, with little or no over oxidation, can be effected by OXONE over either silica gel or alumina in the presence of either liquid CO2 or scCO2.  This occurs despite the fact that amines react rapidly with CO2 to form a carbamic acid salt. We have found that carbamic acid formation is reversible, even in the presence of CO2 at 4000 psi. Substitution of the carbamic acid salt for amine on treatment with OXONE/silica gel in 4000 psi of CO2 affords substantial quantities of the hydroxylamine.  When the oxidation is conducted in the presence of CO2, removal of the hydroxylamine product from the surface of the adsorbent on treatment with methanol is greatly facilitated relative to oxidation conducted in the absence of CO2.  This has now been found to be due to residual CO2 adsorbed on the surface, which reacts with methanol to form the carbonic ester and facilitates desorption of the hydroxylamine as the amine salt.  Removal of methanol was caused decarboxylation and regeneration of the free hydroxylamine. 

 

Electrochemical Methods for Basic Studies in CO2 Media (26)  Murray’s group set out to adapt existing electrochemical microelectrodes and microcells to the CO2 environment. The ionic conductivity strategies include coating the electrode(s) with a swellable ionically conductive film, using ionic fluoro-surfactants as supporting electrolyte in the CO2 "bulk" phase, and using interdigitated array electrodes in steady state mode. An initial working instrument has been assembled for the first measurement targets, which are ionic conductivity of liquid and SCCO2.   Ionic conductivity is a potentially informative property of transport in the CO2 medium, and is an essential ingredient for quantitative voltammetry.   A series of fluorophilic electrolytes and ionic surfactants is undergoing study, both for solubility and for ionic conductivity of their CO2 solutions.   These include variants of siloxane and fluoropolymers terminated with ionic sites like carboxylate and perfluoronated sulfonamide, and which have dissociable counter ions. Both dry and water-containing solutions with measurable and possibly useful ionic conductivities have been found.

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Phase Equilibrium for Polymer/Monomer/Initiator/Supercritical CO2 Systems (19)  Roberts and DeSimone have constructed an apparatus to measure the phase equilibrium between a small molecule dissolved in supercritical CO2 (scCO2) and a polymer that is swollen with scCO2.  Partition coefficients have been measured for the system vinylidene fluoride (VF2)/poly(vinylidene fluoride) (PVDF)/carbon dioxide.  The data are preliminary but show that a substantial quantity of VF2 monomer is present in the CO2-swollen polymer particles during a typical polymerization of VF2 in scCO2.  This is an important issue because the partition coefficient may determine the relative importance of the fluid phase and the polymer phase as loci of polymerization, and may influence the average molecular weight (MW) and MW distribution.  We are beginning to study the solid-state polymerization of poly(bisphenol A carbonate). Pre-polymer has been synthesized with varying concentrations of catalyst and its crystallization with scCO2 is being studied.

 

Novel Polymers and Surfactants (35) DeSimone has focused on synthesis and molecular characterization of two classes of surfactants: cationic silicone surfactants and non-ionic fluoro-polymer surfactants. A series of fluorocarbon block copolymers was synthesized using controlled radical polymerization methods where the second monomer formed a lyophobic, hydrophilic sequence. These molecules stabilize heterogeneous suspension and dispersion polymerizations in CO2. Novel PDMS surfactants having quaternary amine chain end with various anionic counter ions were synthesized and found to be the first cationic surfactants soluble in CO2.  The aggregation behavior of these molecules in CO2 is being studied with collaborators from Thrust Group A, and Murray is exploring the ionic conductivity of solutions of these molecules in CO2.

 

Phase Transfer Catalysis in Liquid and Supercritical CO2 (D1)  P. Tundo (U. of Venice) has begun preliminary investigation on the behavior of scCO2 as a solvent for nucleophilic substitution reactions carried out in the presence of silica supported phase-transfer agents. The nucleophilic displacement on n-octyl methane sulfonate by halide anions (Br-, I-) was chosen as a model reaction. This was conducted according to a novel configuration, wherein the substrate, dissolved in scCO2, was made to react with anionic nucleophiles as counter anions of bulky onium salts anchored to silica gel. The reactivity tests were planned in collaboration between the groups of Tundo and DeSimone and were performed by Dr. Maurizio Selva (Tundo’s group) as a visiting researcher at UNC.  We have demonstrated the following very promising results:

*  in spite of the very low dielectric constant, scCO2 was acceptable for use in reactions involving polar compounds, such as SN2 displacements by anionic nucleophiles; 

*  in the presence of scCO2, supported PT agents exhibited an activity even greater than those of conventional onium salts; 

*  inorganic matrixes (i.e. silica gel) were particularly suitable for these reactions because they have rigid structures that do not swell, unlike poorly designed polymeric supports.

 

Plans for 8/1/000-7/31/01

 

Adewuyi plans to (1) acquire and synthesize novel zeolite catalysts;  (2) characterize  these catalysts using spectroscopic techniques; (3) conduct batch studies using existing facilities at UNC-CH; (4) setup laboratory-scale batch and continuous reactors for catalytic reactions in supercritical CO2 in the Chemical Engineering Department at NCA&T.

            Kelly’s research focus has changed slightly in light of interesting initial results.  Consequently, plans to construct a GC/reactor system will be modified to reflect this “mid-course correction”.  Plans will be finalized by October 2000 after consulting with CERSP leadership.

Paul Kropp plans to study the differences in surface–mediated behavior between liquid CO2 and scCO2 in detail.  In addition, the use of solvent–free conditions for conducting chemical reactions will be studied.  Preliminary studies have shown significant differences.  His studies have also shown that a number of surface–mediated reactions that do not occur in the presence of a solvent proceed readily under solvent–free conditions, especially with accompanying microwave irradiation.  Solvent–free conditions represent an ultimate in pollution prevention and energy efficiency.  The origins of both the greater driving force for solvent–free conditions and enhancement under microwave irradiation will be delineated.

Murray will continue ionic conductivity measurements aiming for an electrolyte medium that permits quantitative microelectrode voltammetry of an electroactive test solute in CO2, such as [Ru(bpy)3]2+ or ferrocene.   Voltammetry will give access to measurements of transport rates and how these are influenced by solvent parameters (pressure, temperature) and by the solvent microstructure (e.g., effects of micellar encapsulation of the redox probe with surfactants).   A second target will be exploration of molecular films (coatings) over the electrodes that are suitable and informative for measurements of intra-film electron or ionic transport and/or for partitioning of redox probes from the CO2 phase.  Swelling and plasticization of the film by CO2 and/or its solutes are expected to be important determinants of transport dynamics.

            DeSimone and Roberts plan to complete the measurement of partition coefficients for the VF2/PVDF/scCO2 system.  Various thermodynamic models, e.g., the SAFT and Sanchez- Lacombe equations of state, will be used to correlate the data in collaboration with Thrust Group B researchers.  The partition coefficient of an initiator mimic, e.g., diethyl carbonate, will be measured.  Equipment to measure the swelling of PVDF in scCO2, and the rate of sorption of various small molecules into PVDF in the presence of scCO2, will be designed and purchased.  The kinetics and equilibrium of the solid-state polymerization of poly(bisphenol A carbonate) will also be characterized quantitatively, as a function of temperature.  An apparatus will be constructed to measure the diffusion coefficient of phenol in poly(bisphenol A carbonate).

            DeSimone will complete the synthesis of a series of cationic PDMS surfactants in the next couple of months.  These molecules will then be thoroughly characterized as to their aggregation behavior in CO2 with and without added water.  Others will study these molecules in the Center using PFG NMR and electrochemical methods.  It is also planned extend the synthetic methodology of making PDMS molecules to include block copolymers that have a labile linkage between the blocks.  The goal would then to use scCO2 to deliver a catalyst to depolymerize the PDMS block to small cyclics which could then be removed leaving nanometer-sized voids which would be useful in a number of applications including nanofilters, interlayer dielectrics, etc. 

P. Tundo will complete construction of an apparatus to use dense CO2. He will identify a set of test reactions using CO2 as a solvent, in the presence of both commercial phase-transfer agents and immobilized PT-catalysts (with diverse salts) over inorganic matrixes such as silica and alumina. He will explore the behavior of the biphasic system in scCO2-H2O

 

Outstanding Achievements